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Eukaryotic cells are characterised by specialised sub-cellular compartments. This compartmental organisation demands that newly synthesised proteins are accurately and efficiently targeted to their appropriate sub-cellular locations. Compartmentalisation also ensures that unique post-translational modifications can occur to a subset of synthesised proteins. The aim of this unit is to examine the molecular mechanisms of protein sorting in eukaryotes, and will review recent data demonstrating that some of these processes are fundamental to all living cells. A substantial part of the course will involve discussion of recently published papers.

 

The aim of this unit is to examine the molecular mechanisms of protein sorting in eukaryotes and will review recent data demonstrating that some of these processes are fundamental to all living cells. A substantial part of the course will involve discussion of recently published papers.

 

Student will be able to:

• explain the mechanisms which target proteins to a number of compartments (including the nucleus, mitochondria and the secretory pathway)
• appraise the maturation of proteins in the endoplasmic reticulum and their subsequent movement through the secretory pathway
• explain the mechanisms of endocytosis and the regulatory role of lipid-mediated signals
• critique the applications and limitations of specific experimental approaches (both classical and ‘cutting edge’) to dissect and understand the different mechanisms of protein sorting
• demonstrate cognitive skills to analyse and critically interpret experimental data and primary papers relating to protein sorting

Protein targeting to the nucleus:

• The nature of nuclear localisation signals and nuclear pores.
• The role of soluble factors in nuclear import.

Protein targeting to mitochondria: 

• Mitochondrial targeting signals.
• Sorting of proteins to specific compartments within mitochondria.

The secretory pathway: 

• Protein targeting to the endoplasmic reticulum.
• Protein translocation into and across the ER membrane.
• Post-translational modification, protein folding and quality control at the ER.

Mechanisms of vesicular transport:

• Formation of transport vesicles.
• Targeting and fusion of transport vesicles.

The endocytic pathway:

• Receptor-mediated endocytosis.
• Ubiquitin-dependent receptor down-regulation.
   

eLearning
There will be a formative ePBL exercise, complementary to the lecture material to further consolidate the course’s experimental/problem-driven approach to the topic of protein sorting.
 

Written exam (based upon a problem question)  70%

Coursework Case Ë®¶à¶àµ¼º½ Exercise  30%

 

This will be via a formative exercise around a data interpretation problem, which will be marked and annotated, feedback to formative PBL exercise, feedback on the coursework assessment via grademark. Feedback on the online written exam will be via general exam feedback summary and feedback clinic.

Recommended Reading
Reference lists of primary and review articles will be given in lectures.

1. Alberts B, Johnson A, Lewis J, Raff M, Roberts K & Walter P, Molecular Biology of the Cell (7th edition), Garland Science/WW Norton, 2022, Recommended
    

The year studying abroad is an integral part of the course and must be completed. Some costs, such as tuition fees, are substantially lower. Other costs can vary and are not included in the tuition fee. For example, living costs may be higher or lower than the UK, and there are travel-related costs to consider, such as paying for a passport, visa fees, health insurance, and (if needed) vaccinations.

All students should normally be able to complete their programme of study without incurring additional study costs over and above the tuition fee for that programme. Any unavoidable additional compulsory costs totalling more than 1% of the annual home undergraduate fee per annum, regardless of whether the programme in question is undergraduate or postgraduate taught, will be made clear to you at the point of application. Further information can be found in the University's (PDF document, 91KB).

Students participating in placements outside the UK may be able to apply for funding from the depending on eligibility. Priority will be given to students from low income households.